Metabolomic studies of saliva in gingivitis and periodontitis

Metabolomic studies of saliva in gingivitis and periodontitis Public Deposited

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Creator

McGuire, Alison
- Affiliation: School of Dentistry, Department of Periodontology

Abstract

The aim of this study was to conduct metabolomic analysis of saliva during 3-week induction and 4-week resolution of stent-induced, biofilm overgrowth (SIBO) in humans to elucidate an association between changes in clinical disease and the salivary metabolome. Five periodontal disease classifications were developed using probing depths and bleeding on probing. SIBO was induced in 50 subjects for 21 days followed by oral hygiene instruction for 28 days. Clinical indices and unstimulated saliva were collected weekly during induction and biweekly during resolution. Samples were analyzed using metabolomic profiling (liquid and gas chromatography mass spectrometry, Metabolon Inc). SIBO was associated with marked, but reversible, increases in clinical indices. 281 metabolites were identified using metabolomic profiling. Eight metabolites demonstrated significant changes including seven in the amino acid super-pathway (threonine, N-acetylserine, serine, 5-oxoproline, histidine, glutamate and erythronate). Analysis of the salivary metabolome may provide new mechanistic insights for periodontal disease progression.

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