microRNAs: Novel Player in Endodontic Disease Public Deposited

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Last Modified
  • March 22, 2019
Creator
  • Zhong, Sheng
    • Affiliation: School of Dentistry, Department of Endodontics
Abstract
  • The purpose of this project was to examine the role of microRNA (miRNA) in endodontic disease. We tested the hypotheses that miRNAs are differentially expressed in infected pulps as compared to normal pulps and that exposure to lipopolysaccharide (LPS) induces changes in macrophage miRNA expression. miRNA expression in normal and infected human pulps (N=28) was profiled using microarray technology. Next CD14 positive monocytes isolated from fresh human buffy coats, and induced towards a macrophage phenotype were challenged with E. coli LPS for up to 8 hours and miRNAs expression was quantified using Nanostring technology. Of the 348 miRNAs expressed in the pulps, 36 were altered in infected pulps (p<0.003). The potential gene targets for these miRNAs included key mediators of the immune response. LPS challenge resulted in time-dependent changes in miRNA expression. Together our data provide support for the role of miRNAs in endodontic disease.
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  • In Copyright
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  • ... in partial fulfillment of the requirements for the degree of Master of Science in the Department of Endodontics, School of Dentistry
Advisor
  • Khan, Asma
Degree granting institution
  • University of North Carolina at Chapel Hill
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