THE EFFECTS OF IONS ON MUCIN RHEOLOGY AND ORGANIZATION

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  • March 20, 2019
Creator
  • Nwachukwu, Kala
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
Abstract
  • Increased mucus thickness is a common symptom of pulmonary diseases, such as cystic fibrosis (CF) and also occurs in chronic cigarette smokers. The thickening of the mucus layer is caused by a defect in cystic fibrosis transmembrane conductance regulator (CFTR), which causes an imbalance of ion transport, leading to reduced CFTR mediated Cl- dehydrated mucus layer, and poor mucociliary clearance. Here, we present data demonstrating the effects of ions that play a role in mucus layer homeostasis and their effects on mucin composition and rheology. We demonstrate that extracellular CaCl2 increases complex mucin viscosity, while MgCl2 has no effect. Furthermore, we show that HCO3- and pH effect mucin rheology, whereas lower pH and higher concentrated HCO3- results in a higher complex viscosity. While the influence of CaCl2 is not currently known, our data suggest that in excess it may contribute to the viscoelastic properties of mucus.
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  • In Copyright
Advisor
  • Cheney, Richard
  • Tarran, Robert
  • Hill, David
  • Otey, Carol
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2016
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