Impact of nanoscale features on implant adherent cell osteoinduction and osteoconduction Public Deposited
- Last Modified
- March 21, 2019
- Creator
-
Alves Daporta, Astrid P.
- Affiliation: School of Dentistry, Department of Prosthodontics
- Abstract
- Objective; To investigate osteogenic and osteoinductive gene expression in cells interacting with modified titanium surfaces in-vitro and an in-vivo. Materials and Methods; Nano-topography was assessed by SEM. hMSC were plated onto cpTi disks (Nano: T1: Acid edged, oxidized; OS: TiO2 fluoride-modified. Micro: T2: Acid etched). RT-PCR was used to assess a wide array of osteoblast specific gene expression (Day 3, 7, 14, 28). Implants were placed in rat tibias (T1, T2, OS and TiOBlast - TiO2 blasted, micro), following harvesting 1, 3 and 7 days after placement. mRNA levels of RUNX-2, ALP and OSX were measured with RT-PCR. Results; In both cell culture and in-vivo experiments, osteoblastic differentiation occurred on all surfaces but to a greater extent on T1 and OS. Conclusions; Presence of nano-features on moderately rough cpTi is associated with marked osteoinduction of adherent hMSC and suggested positive modulation of bone response into the osteogenic pathway in-vivo.
- Date of publication
- May 2011
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- "... in partial fulfillment of the requirements for the degree of Master of Science in the School of Dentistry (Prosthodontics)."
- Advisor
- Cooper, Lyndon F.
- Language
- Publisher
- Place of publication
- Chapel Hill, NC
- Access
- Open access
- Parents:
This work has no parents.
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Impact of nanoscale features on implant adherent cell osteoinduction and osteoconduction | 2019-04-09 | Public |
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