Fluorine-19 (19F) Magnetic Resonance Imaging of Pulmonary Ventilation, Cell Migration, and Inflammation Public Deposited

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  • March 19, 2019
Creator
  • Akinnagbe-Zusterzeel, Esther
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
Abstract
  • Conventional 1H magnetic resonance imaging (MRI) has long been used in a variety of clinical applications. Among other advantages, MRI lacks ionizing radiation while still providing high resolution images with significant soft-tissue contrast. Advances in coil design and pulse sequencing have allowed for the adaptation of clinical scanners to fluorine-19 based MRI (19F MRI). Well-understood to be inert and non-toxic, many commercially available 19F compounds can be introduced into subjects as tracing agents. Resulting images have high signal-to-noise ratios (SNR) due to minimal intrinsic 19F signal; any signal recorded originated from the introduced agent. 19F MRI presents several new opportunities to improve and create applications of an already versatile imaging modality. Pulmonary Imaging As the need to understand the physiology of abnormal pulmonary conditions grows, so does the demand for quantitative lung function imaging methods. The use of exogenous 19F gas along with improved pulse sequencing and gradient hardware has improved the once low-quality lung MR images caused by low water molecule density in lung tissue. This study aimed to demonstrate pulmonary ventilation imaging in healthy controls and cystic fibrosis (CF) patients using 19F MRI to study ventilation kinetics. 19F MR images of twelve adult subjects performing respiratory exercises were acquired using a Siemens TIM Trio 3 Tesla scanner following spirometry. Ventilation kinetics within the lung space were computed by fitting dynamic 19F signal intensity values to a six-parameter fit model. Statistical analysis of ventilation kinetic between groups revealed that CF lungs required significantly more time to fill and showed ventilation defects. 19F imaging presents an inexpensive method of quantitatively measuring lung function in both healthy and affected populations, creating the opportunity to test treatment efficacy of various pulmonary disease as well as structural and physiological lung measurements. Cell Tracking One emerging application of 19F MRI is cell tracking using perfluorocarbon probes (PFC). Human and murine cells were labeled with the PFC probe to explore feasibility of 19F MRI cell tracking and inflammatory response imaging in a CF cell model and murine Parkinson’s disease (PD) model. Studies validated the uptake of the labeling agent by all cell types, showing minimal decrease in cell survival rates. 19F NMR and MRI techniques were employed to analyze loading efficiency and for cell quantification in vivo. We demonstrate preliminary imaging data of fluorine-19 gas and compounds suitable for pulmonary imaging and cell tracking. High signal to noise ratios are achievable with small animal imaging MR scanners as well as conventional 3T human systems with high bandwidth amplifiers.
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  • In Copyright
Advisor
  • Randell, Scott
  • Lalush, David
  • Lee, Yueh
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2017
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