The coeffects of iron and vitamin B12 on marine diatoms Public Deposited

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  • March 19, 2019
Creator
  • Ellis, Kelsey
    • Affiliation: College of Arts and Sciences, Department of Marine Sciences
Abstract
  • Pseudo-nitzschia and Fragilariopsis are two closely related marine diatoms, yet Pseudo-nitzschia possess only the vitamin B12-dependent form of the methionine synthase enzyme [MetH] whereas Fragilariopsis possess both MetH and a B12-independent form [MetE]. Consistent with this difference in gene repertoires, our results indicate that Pseudo-nitzschia granii has an obligate requirement for vitamin B12 while growth of Fragilariopsis cylindrus was unaffected by varying B12 availability due to increased MetE expression. Iron and vitamin B12 can be colimiting in marine high-nutrient, low-chlorophyll [HNLC] regions. Understanding how variations in these nutrients affect diatom growth can further elucidate community succession dynamics in these areas. Our study, conducted with B12-requiring Pseudo-nitzschia granii and MetE-possessing Grammonema cf. islandica, showed that the effects of Fe-B12 colimitation are not synergistic, but rather that Fe effects dominate physiological measures of diatom health regardless of whether a diatom is auxotrophic for vitamin B12.
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  • In Copyright
Advisor
  • Marchetti, Adrian
  • Teske, Andreas
  • Alperin, Marc
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2015
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  • Chapel Hill, NC
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