Detecting DNA Damage: The Synthesis of New Aldehyde Reactive Probes for the Quantitation of Apurinic/Apyrimidinic Sites

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  • March 20, 2019
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  • Ridpath, John R.
    • Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
Abstract
  • Apurinic/apyrimidinic (abasic) sites are some of the most common lesions found in DNA. They are postulated to be intermediates of mutagenicity and carcinogenicity. Each human genome can have as many as 104 abasic sites per day. It is therefore important to be able to quantitate the number of these sites. Abasic sites are quantitated by an aldehyde reactive probe (ARP) assay highly specific for the aldehyde group of the ring-opened d-ribose. This assay is based on formation of a Schiff base adduct of a hydroxylamine on the probe end of the aldehyde. The purpose of this project is to synthesize new ARPs that enhance reactivity without loss of specificity. A two-step synthetic scheme was devised to give a compound which replaces the hydroxylamine group with a hydrazino group expected to be more reactive. A long-chain ARP containing a polyethylene glycol moiety was also synthesized.
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  • In Copyright
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  • Ball, Louise
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