Genipin-modified dentin collagen: Biochemical and biomechanical characterization Public Deposited

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  • March 20, 2019
  • Nagaoka, Hiroko
    • Affiliation: School of Dentistry
  • The objectives of this study were to: 1) characterize genipin (GE)-modified bovine dentin collagen treated with three different doses of GE (0.01%, 0.1% and 0.5%) for several treatment durations as related to changes in its biochemical properties, 2) evaluate changes in the biomechanical properties, as assessed by microtensile bond strength (MTBS), of human dentin collagen that had been modified by four different treatment durations of 0.5% GE. The collagen stability and collagen discoloration assays indicated that the treatment of bovine dentin collagen with GE resulted in a dose- and time-dependent decrease of collagen digestibility and increase of discoloration. Amino acid analysis indicated that the GE-induced cross-links involved the lysine and hydroxylysine residues in dentin collagen. Cross-link analysis showed that GE treatment did not affect the mature cross-link, pyrodinoline; however, dihydroxylysinonorleucine was slightly but significantly decreased (p < 0.05) after 12 h of treatment with GE. MTBS analysis showed that MTBS significantly increased in samples treated with GE for 4 h, 12 h and 24 h when compared to control (p < 0.0001). In addition, MTBS of samples treated with GE for 4 h was significantly higher than those of 12 h and 24 h groups (p < 0.0001). Taken together, these results demonstrate that GE treatment improves the stability of dentin collagen and MTBS, and its potential use for caries prevention and restorative dentistry.
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  • In Copyright
  • Yamauchi, Mitsuo
  • Master of Science
Graduation year
  • 2013

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