Characterisation of Foci Formed by the Telomeric Binding Protein POT-1 in the Developing C. elegans Embryo Public Deposited

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  • March 21, 2019
Creator
  • Peng, Felix
    • Affiliation: School of Medicine, Curriculum in Genetics and Molecular Biology
Abstract
  • The ends of linear chromosomes are protected by telomeres, repetitive DNA caps that are bound by a complex of proteins collectively known as shelterin. POT1, a component of shelterin, binds telomeric single stranded DNA and is important in the regulation of telomeres. Little is known about the regulation of telomeres in the context of early development. Using the early C. elegans embryo as a model, I have developed a system for studying telomeres in live embryos. A transgene expressing POT-1::mCherry exhibits punctate foci at telomeres during early development. Detailed analysis of this transgene in a wild-type and a telomere lengthening background suggest that telomere length may affect POT-1 binding to telomeres.
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  • In Copyright
Advisor
  • Matera, Gregory
  • Ahmed, Shawn
  • Maddox, Paul
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2016
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