VOLUNTARY BINGE-LIKE ETHANOL CONSUMPTION SITE-SPECIFICALLY INCREASES C-FOS IMMUNOEXPRESSION IN MALE C57BL6/J MICE. Public Deposited

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  • March 22, 2019
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  • Burnham, Nathan
    • Affiliation: College of Arts and Sciences, Department of Psychology and Neuroscience
Abstract
  • Forced-ethanol exposure, via intraperitoneal injection (i.p.) or gavage, is commonly used in combination with c-Fos quantification to assess binge-like neuronal activity. Voluntary binge-like ethanol consumption-induced neuronal activity is less understood. Using the “drinking-in-the-dark” (DID) paradigm, we assessed the effects of binge-like ethanol consumption on c-Fos immunoreactivity (IR) in brain regions implicated in ethanol intake and compared this to i.p. ethanol-induced c-Fos. To this end, male C57BL/6J mice were divided into four groups: DID-EtOH (20% v/v), DID-water, i.p.-EtOH (1.5 g/kg), or i.p.-saline (15 mL/kg) and experienced one 4-day DID cycle or a single i.p. injection, respectively, prior to sacrifice. Relative to controls, EtOH groups significantly increased c-Fos IR in several brain regions implicated in neurobiological responses to ethanol. Differences in DID-EtOH and i.p.-EtOH c-Fos were not observed across brain regions examined. These results provide insight into the brain regions that modulate binge-like ethanol intake stemming from DID procedures.
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  • In Copyright
Advisor
  • Reissner, Kathryn
  • Thiele, Todd
  • Lysle, Donald
Degree
  • Master of Arts
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2017
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