Tissue Reservoirs of HIV-1: Insights from the Central Nervous System Public Deposited

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Last Modified
  • March 20, 2019
Creator
  • Rackoff, Lauren
    • Affiliation: School of Medicine, Department of Microbiology and Immunology
Abstract
  • Cellular and anatomical reservoirs of HIV-1 preclude a cure to infection. Efforts to characterize these reservoirs are an important part of developing a strategy to eradicate all forms of HIV-1. The central nervous system (CNS) is a unique bodily compartment that can support viral replication independent of that in the blood (compartmentalization) and may be an anatomical reservoir of unique viruses. In this ‘proof of principle’ study, we characterized viral sequences (RNA and provirus) from the blood, cerebrospinal fluid, brain, and liver of two infected donors who died with HIV-1-associated dementia and disparate states of viral replication (compartmentalized versus equilibrated). We show that selective pressures exist within CNS and liver tissue to drive expansion of particular viral species. We found macrophage-tropic viral lineages archived in the brain, implicating macrophages as a potential cellular reservoir. By including more donors and tissues, our study provides insight towards HIV-1 reservoirs and cure research.
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Rights statement
  • In Copyright
Advisor
  • Swanstrom, Ronald
  • Matsushima, Glenn
  • Moorman, Nathaniel
  • Heise, Mark
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2016
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