Characterization of Recombinant Mouse ecto-5'-nucleotidase

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  • March 22, 2019
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  • Voss, Meagen K.
    • Affiliation: School of Medicine, UNC Neuroscience Center, Neuroscience Curriculum
Abstract
  • Chronic pain is the most common medical complaint in the United States and the number of Americans affected by chronic pain is growing. Effectiveness of available treatments varies greatly and many have intolerable side effects. To address this need for pain treatments, more effective therapies are needed. Recently, our lab discovered an ectonucleotidase in the central nervous system (CNS) called Prostatic Acid Phosphatase (PAP). Spinal injections of PAP into live mice produced antinociceptive effects. The following thesis describes the properties of NT5E, an ectonucleotidase that is colocalized with PAP in the CNS. We have generated a recombinant mouse NT5E protein (mNT5E) and have shown that the protein is pure, catalytically active and capable of reducing nociceptive sensitivity in two animal models of chronic pain. We also show that mNT5E acts through A1-type adenosine receptors (A1R). This study provides an additional target for developing pain treatments and a new tool for studying purinergic signaling.
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  • ... in partial fulfillment of the requirements for the degree of Master of Science in the Curriculum of Neurobiology.
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  • Zylka, Mark J.
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