Development of Real-Time PCR Assays for the Quantitative Detection of Herpesviruses

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  • March 21, 2019
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  • Leopold, Luciana Eleanor
    • Affiliation: School of Medicine, Curriculum in Genetics and Molecular Biology
Abstract
  • Kaposis's sarcoma-associated Herpesvirus (KSHV) and Human Epstein-Barr virus (EBV) are gamma herpesviruses that can cause serious complications in immunocompromised patients. Quantitative detection is critical not only as a diagnostic tool in patients, but also as a method for basic herpesvirology research. Here the development and validation of four real-time PCR assays to quantify viral load of KSHV and EBV in samples is described. The assays are: individual viral load assays for KSHV and EBV, and high-throughput viral load screens for KSHV and EBV. The assays were developed for validation of animal models, drug response studies, basic virology research and medical diagnostics. The data indicate that these are accurate, consistent, and cost-effective assays for the detection of KSHV and EBV infection in various kinds of samples.
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  • In Copyright
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  • Dittmer, Dirk
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  • Open access
Date uploaded
  • October 19, 2010

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