Side Population Sorting Separates Subfractions of Intestinal Stem Cells Public Deposited

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Last Modified
  • March 21, 2019
Creator
  • von Furstenberg, Richard Joseph
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
Abstract
  • The existence of intestinal stem cells (ISCs) which generate the epithelium has been recognized for over three decades. Recent work suggests there are two distinct ISC populations: active ISCs which are important for epithelial homeostasis, and quiescent ISCs that drive proliferation after injury. Although quiescent ISCs may be of greater therapeutic use, to date, isolation of this population has been achieved only with fluorescent reporter mice. My goal was to identify an isolation method for both ISC populations that can be used in wild-type mice and eventually humans. In the following experiments I use side population (SP) sorting to demonstrate that the USP: contains reporter cells of the active ISC, is highly proliferative by S phase analysis, and expresses high levels of the known active ISC transcripts. In contrast the LSP is: non-proliferative, de-enriched for active ISC transcripts, and enriched for those of quiescent ISCs.
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  • In Copyright
Advisor
  • Henning, Susan
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2013
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