Inorganic Arsenic as an Endocrine Disruptor: Modulation of the Glucocorticoid Receptor Pathway and Implications on Placental Physiology Public Deposited

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  • March 22, 2019
Creator
  • Meakin, Cassandra
    • Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
Abstract
  • Prenatal exposure to inorganic arsenic (iAs) has been associated with adverse developmental and reproductive outcomes, and may be tied to altered glucocorticoid receptor (GR) in the placenta. In this study, we investigated whether iAs exposure modulates GR signaling in placental cells. Trophoblast JEG-3 cells were exposed to environmentally-relevant doses of iAs and mRNA expression and DNA methylation were quantified. Results demonstrated that iAs exposure alters the expression of 12 GR-genes that play a role in fetal and placental development. Furthermore, supporting a role for the epigenome in control of this expression, the mRNA alterations were associated with changes in DNA methylation patterning in placental cells. The identified target genes have been implicated in associations with prenatal arsenic exposure, placental physiology, and fetal development. This study provides evidence of iAs as an endocrine disruptor and insight into the mechanisms by which prenatal iAs exposure induces adverse birth outcomes.
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  • In Copyright
Advisor
  • Fry, Rebecca
  • Stewart, Jill
  • Nylander-French, Leena
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2018
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