Modeling Cell-Extracellular Matrix Interactions: Spatial and Conformational Control of Ligand Presentation on Self-Assembled Monolayers Public Deposited

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  • March 22, 2019
Creator
  • Lamb, Brian M.
    • Affiliation: College of Arts and Sciences, Department of Chemistry
Abstract
  • My doctoral research at the University of North Carolina at Chapel Hill has focused on developing tools for controlling the spatial and conformational presentation of surface-bound ligands and biomolecules to cells. Technological developments in this area are important for modeling normal cellular functions and will enable a better understanding of the dynamic processes that perpetually occur between a cell and its surrounding extracellular matrix. The current needs of cell and cancer biologists are twofold: 1.) a simple method for controlling and orienting intracellular processes, such as cell migration, 2.) a solution-orthogonal (reagent free) approach for actuating biomolecule conformation and binding affinity. I developed an inexpensive and effective technique for patterning haptotactic gradients that is capable of directing cell polarization, migration, and orienting cell mitosis to address these needs. Furthermore, a molecular strategy for reversibly controlling biomolecule conformation via electrochemical modification in the presence of cells was realized. Applications of this method for actuating cell adhesion and migration via an electrochemical switch were investigated. Together, both methods enable the control of complex cellular processes and will increase scientific understanding of cancer and disease etiology.
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  • In Copyright
Advisor
  • Yousaf, Muhammad
Degree
  • Doctor of Philosophy
Graduation year
  • 2011
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