DEVELOPMENT OF A LINE-FIELD MAGNETO-MOTIVE OPTICAL COHERENCE TOMOGRAPHY SYSTEM Public Deposited

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Creator
  • Barrick, Jessica
    • Affiliation: College of Arts and Sciences, Department of Physics and Astronomy
Abstract
  • The mechanism by which certain species of animals are able to detect the Earth’s magnetic field has remained a mystery for as long as we have known that they exhibit geomagnetic navigation. Certain species of bacteria are known to contain single chains of magnetite crystals, each with a diameter of ~50 nm, that are used to orient the bacteria. Searching for similar magnetoreceptors in larger animals requires a high-speed, high-resolution imaging system with the ability to detect single magnetic nanoparticles. Optical coherence tomography (OCT) is a biomedical imaging modality that produces 2D, cross-sectional images of optically turbid media with a resolution on the order of 1-10 µm. Magneto-motive OCT (MMOCT) is a functional form of OCT that can detect the sub-resolution displacement of magnetic nano- or micro-particles embedded in weakly diamagnetic, optically scattering, elastic media (such as human and animal tissues) subject to a sinusoidally-varying magnetic gradient force. This dissertation describes the design and implementation of an MMOCT system composed of a novel combination of a line-field configuration with a supercontinuum light source and a faster MMOCT imaging scheme. The combination of the line illumination with a high-speed 2D camera and the low-noise, high-power supercontinuum light source produces the best combination of axial resolution, optical SNR, and imaging speed of any line-field-OCT (LFOCT) system to date. The performance of the LF-OCT system combined with the faster magnet modulation scheme results in a LF-MMOCT system with a volumetric imaging speed comparable to that of the highest speed MMOCT system to date. High volumetric imaging speed is essential for the problem of endogenous magnetite detection, as is high magnetic sensitivity. The LF-MMOCT system is optimized to produce the best possible magnetic SNR at kilohertz framerates. We then demonstrate the detection of single magnetic point particles, measure the vibration amplitude produced by an external magnetic gradient force on each point particle, and compare that vibration amplitude to a theoretical value. The ability to image a single magnetic point particle with a high-resolution, high-sensitivity, and high-speed LF-MMOCT system provides a key proof of concept that this system may be used for endogenous magnetite detection.
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Advisor
  • Oldenburg, Amy L.
  • Superfine, Richard
  • McNeil, Laurie E.
  • Mersini-Houghton, Laura
  • Branca, Tamara
Degree
  • Doctor of Philosophy
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2020
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