Exploring the Relationships among the Placental and Umbilical Microbiomes, the Oral Microbiota, and Adverse Pregnancy Outcomes Public Deposited

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  • March 20, 2019
Creator
  • Reyes Viruet, Krystal
    • Affiliation: School of Dentistry, Department of Periodontology
Abstract
  • Aims: To compare the microbiomes of placenta and umbilicus samples from 44 women presenting periodontal diseases who experienced full term (FTB, n=22) and preterm births (PTB; <35 weeks, n=22), as well as, the level of maternal subgingival periodontal species in each group pre and post-partum. Materials and methods: Clinical and demographic data, subgingival plaque samples, as well as, placenta and umbilical cord tissues samples were obtained. The microbial content of the tissue samples were analyzed using 16rRNA sequencing, while subgingival biofilm samples were analyzed using checkerboard DNA-DNA hybridization method. Results: Most abundant phylum in both placenta and umbilicus samples was Firmicutes. Most abundant genera in both placenta and umbilicus samples was Veillonella. Some of the most common species detected were Neisseria elongata, Pyramidobacter piscolens, and Gemella morbillorum. Conclusion: PTB and FTB exhibit distinct umbilical microbiomes. PTB samples are more heavily colonized by oral pathogenic taxa, whereas FTB specimens had higher levels of commensal organisms.
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  • In Copyright
Advisor
  • Offenbacher, Steven
  • Azcarate-Peril, Andrea
  • Teles, Flavia
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2017
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