Design of a Nanoscale Time-of-Flight Sensor and an Integrated Multiscale Module for the Point-of-Care Diagnosis of Stroke

Design of a Nanoscale Time-of-Flight Sensor and an Integrated Multiscale Module for the Point-of-Care Diagnosis of Stroke Public Deposited

Last Modified

Creator

Andrus, Matthew
- Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering

Abstract

Stroke is a leading cause of death and disability in the United States, however, there remains no rapid diagnostic test for differentiating between ischemic and hemorrhagic stroke within the three-hour treatment window. Here we describe the design of a multiscale microfluidic module with an embedded time-of-flight nanosensor for the clinical diagnosis of stroke. The nanosensor described utilizes two synthetic pores in series, relying on resistive pulse sensing (RPS) to measure the passage of molecules through the time-of-flight tube. Once the nanosensor design was completed, a multiscale module to process patient samples and house the sensors was designed in a similar iterative process. This design utilized pillar arrays, called "pixels" to immobilize oligonucleotides from patient samples for ligase detection reactions (LDR) to be carried out. COMSOL simulations were performed to understand the operation and behavior of both the nanosensor and the modular chip once the designs were completed.

Date of publication