Design of a Nanoscale Time-of-Flight Sensor and an Integrated Multiscale Module for the Point-of-Care Diagnosis of Stroke Public Deposited

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Last Modified
  • March 19, 2019
Creator
  • Andrus, Matthew
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering
Abstract
  • Stroke is a leading cause of death and disability in the United States, however, there remains no rapid diagnostic test for differentiating between ischemic and hemorrhagic stroke within the three-hour treatment window. Here we describe the design of a multiscale microfluidic module with an embedded time-of-flight nanosensor for the clinical diagnosis of stroke. The nanosensor described utilizes two synthetic pores in series, relying on resistive pulse sensing (RPS) to measure the passage of molecules through the time-of-flight tube. Once the nanosensor design was completed, a multiscale module to process patient samples and house the sensors was designed in a similar iterative process. This design utilized pillar arrays, called "pixels" to immobilize oligonucleotides from patient samples for ligase detection reactions (LDR) to be carried out. COMSOL simulations were performed to understand the operation and behavior of both the nanosensor and the modular chip once the designs were completed.
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  • In Copyright
Advisor
  • Walker, Glenn
  • Taylor, Anne
  • Soper, Steven
  • Ligler, Frances
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2015
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  • Chapel Hill, NC
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