Antibacterial Effects of Ozone on Streptococcus mutans

Antibacterial Effects of Ozone on Streptococcus mutans Public Deposited

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Creator

Astorga, Fernando
- Affiliation: School of Dentistry, Department of Operative Dentistry

Abstract

Aims: To investigate the antibacterial effects of ozone on Streptococcus mutans in vitro. Methods: A bacteria volume of 106 CFU/ml was inoculated on human and bovine dentin specimens and in Wilkins-Chalgren broth media (WC, Difco Laboratories. Sparks) treated with two ozone sources (Curozone X4®, Kavo; OL80A and OL80W, Yanco Industries), at various concentrations (2, 20, 200, 2000 and 2100 ppm) and exposure times (from 15 sec to 4 min). Results: Curozone X4 had no effect on bacteria recovery, while OL80A and OL80W showed a concentration-dependent effect on S. mutans recover on a Trypticase™ Soy Agar with 5% Sheep Blood (Difco Laboratories. Sparks). Concentrations of 200 and 2000 ppm showed no bacteria recovery after treatment for 4 min, while 2 and 20 ppm showed significant reduction of the recovered bacteria. Conclusions: This study suggests that Curozone X4® generator is not capable of delivering ozone to would effectively disinfect a caries lesion.

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