THE ROLE OF AUTOPHAGY DURING ORTHODONTIC TOOTH MOVEMENT

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  • March 22, 2019
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  • Li, Yina
    • Affiliation: School of Dentistry, Department of Orthodontics
Abstract
  • Orthodontic tooth movement (OTM) depends on efficient remodeling of surrounding alveolar bone. While a well-controlled inflammatory response is essential during such biological processes, the precise mechanism by which how inflammation is regulated hasn’t been fully understood. Autophagy, a conserved catabolic pathway, has been shown to protect cells from excessive long lasting inflammation in nervous systems and other disease conditions. We hypothesize that autophagy plays a role in regulating inflammation during OTM. By using a split mouth design in adult male mice at different time points (days 0, 1, 3, 5, 7, 10 and 14) after 30 gram of force loading, we found that autophagy activity increased shortly after loading (as early as day 1) and was closely associated with inflammatory cytokine expression as well as osteoclast activation (by TRAP staining). Autophagy activation appeared to be at the protein, not mRNA, level. Daily administration of rapamycin, autophagy activator, in adult male mice led to reduced tooth movement amount as well as inflammatory signal after loading, suggesting a negative effect of autophagy on inflammatory response during OTM. To our knowledge, this is the first time that research showed autophagy plays a role during orthodontic tooth movement, likely via negative regulation of inflammatory response. More molecular and cellular analyses are needed to elucidate the underlying mechanism that governs the regulation of inflammation by autophagy pathway.
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Advisor
  • Tseng, Henry
  • Martinez, Jennifer
  • Ko, Ching-Chang
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2018
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