Molecular assessment of endosseous implant adherent cellular phenotypes in man Public Deposited

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  • March 21, 2019
Creator
  • Bryington, Matthew Scott
    • Affiliation: School of Dentistry, Department of Prosthodontics
Abstract
  • This investigation considered the role of implant surface roughness on activation of osteoblastic differentiation of uncommitted mesenchymal stem cells in humans. The aim of this study was to examine the gene expression profiles from adherent cells on moderately roughened TiO2 grit-blasted (micron-scale topography) and hydrofluoric acid modified TiO2 grit-blasted (nano-scale topography) implants. Sixty cpTitanium implants were placed and allowed to integrate for 24 hours, 72 hours, and one week in 10 systemically healthy patients. These implants were retrieved and biochemical analyzed by RT-PCR for markers of osteoblastic differentiation. A complex array of gene changes representative of osteogenesis was observed at both implant surfaces. Compared to implants with micron-scale topography, nano-scale topography implants supported greater expression of RUNX-2(1.6 vs. 2.6-fold,p=0.18), Osterix(1.8 vs. 3.9-fold,p=0.04), BSP(2.7 vs. 4.6-fold, p=0.11) and OCN(1.6 vs. 3.2-fold,p=0.06) mRNAs indicating greater osteogenesis at 7 days in vivo. Further investigations using this model may elucidate important difference among subsets of patients who experience implant failure.
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  • In Copyright
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  • "... in partial fulfillment of the requirements for the degree of Masters of Science in the School of Dentistry (Prosthodontics)."
Advisor
  • Cooper, Lyndon F.
Degree granting institution
  • University of North Carolina at Chapel Hill
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Place of publication
  • Chapel Hill, NC
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  • Open access
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