Regulation of Membrane Traffic by Intrinsic and Extrinsic Mechanisms

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Hung, Chao-Wei
- Affiliation: College of Arts and Sciences, Department of Biology

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Vesicular membrane traffic regulates many biological activities, such as cell growth, motility and the maintenance of the cell shape. One of the most important types of traffic is mediated by clathrin coated vesicles. To form a vesicle, clathrin must be recruited to the membranes where cargoes are located by clathrin adaptors. Although it is well established that clathrin adaptors are essential for the recruitment of clathrin and the initiation of traffic, not much is known about the mechanisms by which cells regulate the recruitment and activities of adaptors. Furthermore, the role of clathrin adaptors in traffic beyond simple clathrin recruitment remains largely unexplored. In this dissertation, I explored the regulation of adaptor activities and recruitment by intrinsic regulatory motifs and extrinsic energy dependent mechanisms. I revealed the motifs on adaptors Gga2 and Ent5 that are important for the temporal regulation of adaptor recruitment. I also demonstrated a multi-step, energy dependent mechanism that regulates the recruitment of adaptors in response to the energy availability. Finally, we revealed that in addition to recruiting clathrin and initiating traffic, clathrin adaptors are also involved in the late stage of traffic. These findings suggest the role of adaptors in traffic is more diverse.

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