Comparative Analysis of Epigenetic and Gene Expression Endpoints Between Tumorous and Non-tumorous Tissues from HCV-positive Patients with Hepatocellular Carcinoma Public Deposited
- Last Modified
- March 20, 2019
- Creator
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Formeister, Eric J.
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Transcriptional silencing induced by promoter CpG island hypermethylation is an important epigenetic mechanism of hepatocarcinogenesis. The goals of our study were to examine promoter methylation and mRNA levels of candidate genes, as well as global changes in DNA methylation, in a cohort of HCV-positive HCC patients from Japan. Methylation-specific PCR was used to assess the methylation status of seven cancer-related genes, while the methylation status of long interspersed nuclear elements was used as marker of global genomic methylation, in tissues obtained from patients who underwent tumor resection surgery. Methylation frequencies for most of the genes were significantly higher in tumorous versus non-tumorous tissues. The methylation status of only three genes correlated with reduced mRNA levels. Genomic DNA was significantly more hypomethylated in tumorous tissues, and was associated with shorter recurrence but not with clinicopathological variables. In summary, this study establishes an aberrant gene-specific and global methylation profile in HCV-associated HCCs.
- Date of publication
- May 2010
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- "... in partial fulfillment of the requirements for the degree of Master of Science in the Gillings School of Global Public Health, Department of Environmental Sciences and Engineering."
- Advisor
- Rusyn, Ivan
- Language
- Publisher
- Place of publication
- Chapel Hill, NC
- Access
- Open access