Concentration of Adenoviruses and Noroviruses from Seawater with Argonide Nanoceram Cartridge Filters: Method Effectiveness and Occurrence in Southern California Recreational Waters

Concentration of Adenoviruses and Noroviruses from Seawater with Argonide Nanoceram Cartridge Filters: Method Effectiveness and Occurrence in Southern California Recreational Waters Public Deposited

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Gibbons, Christopher
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering

Abstract

The goals of this study were to evaluate Argonide Nanoceram cartridge filters for concentrating adenoviruses and noroviruses from seawater and then apply them to Southern California recreational water samples. The filters were challenged with 20-40 L of seawater seeded with test viruses; the recoveries of adenoviruses and noroviruses were 3.2% (± 2.3%) and 111% (± 28%), respectively. PEG precipitation was used as a secondary concentration step, and recovery rates with optimized conditions were 34% (± 12%) for adenovirus, and 59% (± 4.8%) for norovirus. These methods were applied to seawater samples from two Southern California beaches for the detection of both viruses. For Doheny Beach, 24 and 21 of 93 samples were positive for adenovirus and norovirus, respectively. For Avalon Beach, 26 and 2 of 286 samples were positive for adenovirus and norovirus, respectively. The presence of enteric viruses was not associated with the presence of coliphages in this study.

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