Examination of selective broth culture media for the detection and quantification of Vibrio cholerae in drinking water using an adapted Compartment Bag Test

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  • March 21, 2019
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  • Lott, Megan
    • Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
Abstract
  • Cholera outbreaks may be mitigated by monitoring drinking waters for the bacterial disease agent, Vibrio cholerae. Present microbial methods for V. cholerae require advanced training and specialized equipment; these methods may not be appropriate for low-resource settings most vulnerable to cholera outbreaks. Our team hypothesized that the Aquagenx Compartment Bag Test (CBT) kit could be adapted for the direct detection and quantification of V. cholerae in drinking water in field settings. The present study evaluates selective culture media for adapted field use. The results of this study suggest that previously-described and commercially-available media are limited in specificity, exclusivity, and plating efficiency. We propose new selective broth culture media for use with the CBT. Preliminary results suggest that newly-proposed media demonstrate improved exclusivity over other existing culture media. These results support the further examination of broth media for direct quantification of V. cholerae with an adapted Cholera CBT.
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  • In Copyright
Advisor
  • Stewart, Jill
  • Abebe, Lydia
  • Sobsey, Mark
Degree
  • Master of Science
Degree granting institution
  • University of North Carolina at Chapel Hill Graduate School
Graduation year
  • 2018
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