Novel Colorimetric Technique for the Detection of Pathogenic Vibrio vulnificus
Public Deposited- Last Modified
- February 26, 2019
- Creator
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Lorentz, Alexandra
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Vibrio vulnificus infections are expected to continue to increase with climate change yet current detection methods are either too expensive for wide spread use or unable to distinguish between strains likely to cause disease and those that will not. This study investigates the use of serum and mannitol to develop a culture-based detection method for pathogenic strains. Two genes correlated with virulence were used as indicators of pathogenicity, vcg and pilF. The medium developed contains 30% serum and 70% mannitol broth, and has accuracies in detecting pathogenic strains of V. vulnificus as high as 74% when pilF is used as the indicator of pathogenicity.
- Date of publication
- spring 2015
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: None
- Advisor
- Noble, Rachel T.
- Degree
- Bachelor of Science
- Honors level
- Highest Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Extent
- 13
- Date uploaded
- April 24, 2015
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