The Role of Glucose in the Response to the Influenza Virus: Does Hyperglycemia Affect Neutrophil Function and Susceptibility to Influenza Infection?

Public Deposited
Analytics

Downloadable Content

Download PDF
Request Version for Screen Reader
Last Modified
  • February 26, 2019
Creator
  • Garba, Amina
    • Affiliation: Gillings School of Global Public Health, Department of Nutrition
Abstract
  • Background: Obesity-induced hyperglycemia as seen in type 2 diabetes, may predispose people to impaired immune function dysregulated neutrophils, which will then increase their susceptibility to influenza virus infection. Objective: My goal was to study the effect of obesity-induced abnormal glucose metabolism on neutrophil function and to determine whether neutrophil function and activation is affected by exposure to glucose at hyperglycemic levels. I hypothesized that hyperglycemia will have a strong effect on the ability of neutrophils to function and will cause their dysregulation, displayed by a lower generation of influenza virus titers and higher cell counts in hyperglycemic conditions. Design: HL-60 cells were grown, differentiated into neutrophils, and infected with 1, 10, and 100 HAU of PR8/H1N1 influenza virus in different glucose concentrations for 24 hours. Post-infection, morphological changes were observed, cell viability was assessed, and a TCID50 assay was conducted to determine infectious virus titers. Results: No significant link was found between hyperglycemia and dysregulated neutrophils (HL-60 cells). Glucose, therefore, had no effect on the ability of HL-60 cells to grow or the TCID50 titers. There was a significant link in the amount of virus used. 100 HAU killed more cells than 10 HAU and 1 HAU and there was a significant difference in the amount of growth with and without virus exposure. Additionally, there was a significant difference in the TCID50’s depending on if the virus concentration was 1 HAU, 10 HAU or 100 HAU, except for between 1 HAU and 10 HAU. Conclusion: Future research regarding a link between hyperglycemia and neutrophil dysregulation should be conducted.
Date of publication
Keyword
DOI
Resource type
Rights statement
  • In Copyright
Note
  • Funding: None
Advisor
  • Beck, Melinda A.
Degree
  • Bachelor of Science in Public Health
Academic concentration
  • Nutrition
Honors level
  • Honors
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2017
Language
  • English

Relations

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details 2017_Garba.pdf 2019-05-17 Public Download