A Paper-Based Transwell Assay to Study Cancer-Cell Invasion in 3D Public Deposited

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Last Modified
  • February 26, 2019
Creator
  • Loeser, Adam
    • Affiliation: College of Arts and Sciences, Department of Chemistry
Abstract
  • Cellular invasion is the gateway to metastasis, the leading cause of cancer-related deaths today. Therefore, in the fight against cancer, it is crucial to better understand the cues and conditions under which cellular invasion occurs. Such an understanding has the potential to lead to therapeutic treatments capable of halting a tumor in its progression towards a metastatic state. A popular current method of studying invasion is the modified Transwell assay, comprised of two chambers separated by a matrix-coated membrane through which cells can migrate in response to various chemokines. Although Transwell assays are readily available, customizable and provide easily interpretable data, they are expensive and offer little flexibility in assay setup. Here, we present a paper-based variation of the Transwell assay capable of generating similar datasets with equal reproducibility to the traditional setup at a fraction of the cost.
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Rights statement
  • In Copyright
Advisor
  • Lockett, Matthew
Degree
  • Bachelor of Arts
Academic concentration
  • Chemistry
Honors level
  • Honors
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2018
Language
  • English
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