Metabolite Accumulation During m-Cresol Degradation by Aquatic Bacteria Public Deposited

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Last Modified
  • February 28, 2019
Creator
  • Most, Claire Caughey
    • Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
Abstract
  • The accumulation of metabolites in the aqueous phase was measured during studies of m-cresol biodegradation with natural water samples. 14C-m-cresol was separated from radiolabeled products remaining in the aqueous filtrates using HPLC. In heterotrophic activity studies, which involve short-term incubations (< 24 h), metabolite accumulation accounted for only a small fraction of the radiolabeled m-cresol originally added. After longer incubations characteristic of die-away studies, metabolite accumulation accounted for as much as 30% of the label originally added. The nature of metabolite accumulation varied, and appeared to reflect differences in the initial environmental samples. Sometimes no metabolite accumulation was detected. One time, a few products accumulated and they were less polar than m-cresol. At other times, many different products accumulated and they were more polar than m-cresol. When metabolite accumulation occurred, it was greater in samples receiving 960 ug/1 m-cresol than 120 ug/1 m-cresol. No one metabolite accumulated consistantly to a level greater than 5% of the radiolabel originally injected.
Date of publication
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Rights statement
  • In Copyright
Advisor
  • Sobsey, Mark
  • Pfaender, Frederic K.
  • Turner, Alvis G.
Degree
  • Master of Science in Public Health
Academic concentration
  • Aquatic and Atmospheric Sciences
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 1985
Language
Deposit record
  • 4229e0fb-b010-4ede-aa50-9d4a2f0726fb
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