Viability of Cultured Primary Human Skin Cells Treated with 1,6-Hexamethylene Diisocyanate Monomer and its Oligomer Isocyanurate Public Deposited

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Last Modified
  • February 28, 2019
Creator
  • Phillips, Kaitlyn
    • Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
Abstract
  • The diisocyanate monomer 1,6-hexamethylene diisocyanate (HDI) and its oligomer HDI isocyanurate are components in sprayed polyurethane coatings. Exposure via the lungs and skin can lead to allergic sensitization and asthma. Research on these compounds has focused on effects of exposure on respiratory and immune cells and variation in gene expression, pathway activation, and mechanisms influencing toxic response. Here, we focused on the toxic effects of HDI monomer and HDI isocyanurate on three types of cultured primary human skin cells, namely keratinocytes, melanocytes, and fibroblasts. To determine the cell-type specific toxicity, we used a luminescent ATP-viability assay. The dose-response data indicated that sensitivity to death varied among the different skin cell types and death by necrosis. The observed variations in toxicity between the HDI monomer and HDI isocyanurate as well as between the cell types may have important implications for developing an adverse effect, for regulatory limits, and for worker safety.
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Rights statement
  • In Copyright
Advisor
  • Nylander-French, Leena A.
Reviewer
  • Jaspers, Ilona
  • Fry, Rebecca
Degree
  • Master of Science in Public Health
Academic concentration
  • Environmental Health Sciences
Degree granting institution
  • University of North Carolina at Chapel Hill
Graduation year
  • 2017
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Deposit record
  • 4ce3ed5d-5a25-4f27-9cef-a06a86f48c63
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