A Comparison of Speciation Methods on Environmentally Isolated, Sediment Associated Aeromonas using Matrix Assisted Laser Desorption/Ionization Mass Spectrometry and Three Housekeeping Genes
Public Deposited- Last Modified
- February 28, 2019
- Creator
-
Huslage, Daniel Kirk
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- Background: The taxonomy of Aeromonas is complex and has confounded researchers for years. Over the past twenty years, technological advances have allowed researchers to attempt to clarify the taxonomy through genetic and proteomic techniques. Several of these advances include PCR amplification and sequencing of numerous housekeeping genes and small sub-unit ribosomal DNA (16SrDNA) in order to determine the small taxonomic differences between the species in the genus Aeromonas. Another method that has recently been employed in this endeavor is matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). Since it is a relatively new method used for aeromonads, no one to date has sought to compare the genetic and proteomic approaches to see which has more utility for the determination of species within the genus.
- Date of publication
- December 2007
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Stamm, Lola
- Sobsey, Mark
- Simmons, Otto D.
- Ball, Louise
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 2007
- Language
- Deposit record
- e00f1df6-6c31-41ba-867b-150d86a8c8cb
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