Affiliation: College of Arts and Sciences, Department of Biology
Other Affiliation: Lineberger Comprehensive Cancer Center
The Pecot Lab has discovered sequences for antisense oligonucleotides (ASOs) that can target the four most common mutations of KRAS (G12C, G12D, G12V, and G13D). We may utilize this sequence to synthesize an ASO that can effectively target mutated KRAS while sparing wild-type KRAS in healthy tissue to reduce potential toxic side-effects. Our project investigated the efficacy of EFTX-016 ASO to inhibit KRAS expression by investigating the cellular uptake and knockdown capability of our ASO sequences.