The effects of substrate stiffness on non-canonical Notch signaling Public Deposited

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  • September 30, 2019
  • Young, Zachary
    • Affiliation: School of Medicine, UNC/NCSU Joint Department of Biomedical Engineering - Biomedical Engineering
  • Vascular barrier dysfunction is a major health complication and is associated with ischemia and cardiovascular disease. In a recent Nature paper, Polacheck et al. discovered that the Notch receptor in vascular endothelial cells plays a major role in a novel, force sensitive signaling pathway that regulates vascular barrier function. We asked whether activation of the NCNP by Notch ligand DLL4 changes due to a difference in cell generated forces produced by growing vascular endothelial cells on polyacrylamide hydrogels of different stiffnesses. Overall, we found differences in phenotype based on the stiffness of the gel. There was no significant difference in Notch activation between our control group without DLL4 and our experimental group with DLL4.
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