Characterization of the Novel Fluoride Resistant Gene flr-3 in C. elegans

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  • August 25, 2022
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  • Honey, Kendra
    • Affiliation: School of Medicine, Department of Cell Biology and Physiology
Abstract
  • The nematode Caenorhabditis elegans is a simple model organism used to better understand the mechanistic basis of human health and disease, as most cellular, molecular, and genetic pathways are conserved to humans. We have identified DRL-1 as a Mitogen Activated Protein (MAP) kinase responsible for proper growth and lipid homeostasis in C. elegans. The flr- 3 mutation, which was identified in a forward genetic screen, has been previously shown to confer fluoride resistance, however the molecular identity of flr-3 remains unknown. To investigate the function of flr-3, we examined its role in development and lipid reallocation, as well as compared flr-3 mutant phenotypes to the drl-1 mutant. Our results indicated that flr-3 genocopies drl-1, displaying defects in growth and lipid reallocation, indicating that it may function within the same pathway. Additionally, a complementation cross revealed that flp-2, a known mutation in the flr-3 mutant strain, does not the cause the flr-3 mutant phenotypes, leaving the genetic identity of flr-3 unknown.
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