Developing Experimental Tools to Enable Further Study in Tardigrades

Public Deposited

Downloadable Content

Download file
Request Version for Screen Reader
Last Modified
  • September 30, 2019
Creator
  • Shaljian, Emily
Abstract
  • In this project, I worked to genetically manipulate the tardigrade species Hypsibius exemplaris as a means towards developing viable transgenic tardigrades. By observing the eye pigmentation pathways in the related organism Drosophila melanogaster and finding homologs of the involved genes in Hypsibius, I developed a list of target genes for RNA interference. I designed primers for the target genes, amplified them by PCR, and translated them to make dsRNAs, which were then injected into the adult tardigrade. Offspring of injected adults were observed for loss of eye pigments. This project also involved experiments to incorporate mRNA into the the body cavity of an injected adult tardigrade.
Date of publication
DOI
Rights statement
  • In Copyright
Language

Relations

Parents:

This work has no parents.

In Collection:

Items

Thumbnail Title Date Uploaded Visibility Actions
file details 2019_Shaljian_Emily_SURFPowerPoint.pptx 2019-09-30 Public Download