Developing Experimental Tools to Enable Further Study in Tardigrades

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  • September 30, 2019
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  • Shaljian, Emily
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  • In this project, I worked to genetically manipulate the tardigrade species Hypsibius exemplaris as a means towards developing viable transgenic tardigrades. By observing the eye pigmentation pathways in the related organism Drosophila melanogaster and finding homologs of the involved genes in Hypsibius, I developed a list of target genes for RNA interference. I designed primers for the target genes, amplified them by PCR, and translated them to make dsRNAs, which were then injected into the adult tardigrade. Offspring of injected adults were observed for loss of eye pigments. This project also involved experiments to incorporate mRNA into the the body cavity of an injected adult tardigrade.
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