Global identification of bursicon-regulated genes in Drosophila melanogaster
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An, Shiheng, et al. Global Identification of Bursicon-regulated Genes In Drosophila Melanogaster. BioMed Central Ltd, 2008. https://doi.org/10.17615/qk6t-ec58APA
An, S., Wang, S., Gilbert, L., Beerntsen, B., Ellersieck, M., & Song, Q. (2008). Global identification of bursicon-regulated genes in Drosophila melanogaster. BioMed Central Ltd. https://doi.org/10.17615/qk6t-ec58Chicago
An, Shiheng, Songjie Wang, Lawrence I Gilbert, Brenda Beerntsen, Mark Ellersieck, and Qisheng Song. 2008. Global Identification of Bursicon-Regulated Genes In Drosophila Melanogaster. BioMed Central Ltd. https://doi.org/10.17615/qk6t-ec58- Creator
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An, Shiheng
- Other Affiliation: Division of Plant Sciences, University of Missouri
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Wang, Songjie
- Other Affiliation: Division of Plant Sciences, University of Missouri
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Gilbert, Lawrence I
- Affiliation: College of Arts and Sciences, Department of Biology
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Beerntsen, Brenda
- Other Affiliation: Department of Veterinary Pathobiology, University of Missouri
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Ellersieck, Mark
- Other Affiliation: Experiment Station Statistics, University of Missouri
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Song, Qisheng
- Other Affiliation: Division of Plant Sciences, University of Missouri
- Abstract
- Background Bursicon is a heterodimer neuropeptide responsible for regulating cuticle sclerotization and wing expansion in several insect species. Recent studies indicate that the action of bursicon is mediated by a specific G protein-coupled receptor DLGR2 and the cAMP/PKA signaling pathway. However, little is known regarding the genes that are regulated by bursicon. The identification of bursicon-regulated genes is the focus of this investigation. Results We used DNA microarray analysis to identify bursicon-regulated genes in neck-ligated flies (Drosophila melanogaster) that received recombinant bursicon (r-bursicon). Fifty four genes were found to be regulated by bursicon 1 h post r-bursicon injection, 52 being up-regulated and 2 down-regulated while 33 genes were influenced by r-bursicon 3 h post-injection (24 up-regulated and 9 down-regulated genes). Analysis of these genes by inference from the fly database http://flybase.bio.indiana.edu revealed that these genes encode proteins with diverse functions, including cell signaling, gene transcription, DNA/RNA binding, ion trafficking, proteolysis-peptidolysis, metabolism, cytoskeleton formation, immune response and cell-adhesion. Twenty eight genes randomly selected from the microarray-identified list were verified by real time PCR (qPCR) which supported the microarray data. Temporal response studies of 13 identified and verified genes by qPCR revealed that the temporal expression patterns of these genes are consistent with the microarray data. Conclusion Using r-bursicon, we identified 87 genes that are regulated by bursicon, 30 of which have no previously known function. Most importantly, all genes randomly selected from the microarray-identified list were verified by real time PCR. Temporal analysis of 13 verified genes revealed that the expression of these genes was indeed induced by bursicon and correlated well with the cuticle sclerotization process. The composite data suggest that these genes play important roles in regulating the cuticle sclerotization and wing expansion processes. The data obtained here will form the basis for future studies aimed at elucidating the exact mechanisms upstream from the secretion of bursicon and its binding to target cells.
- Date of publication
- September 18, 2008
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- Shiheng An et al.; licensee BioMed Central Ltd.
- License
- Journal title
- BMC Genomics
- Journal volume
- 9
- Journal issue
- 1
- Page start
- 424
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1471-2164
- Bibliographic citation
- BMC Genomics. 2008 Sep 18;9(1):424
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- August 23, 2012
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