Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro

Public Deposited
Analytics

Downloadable Content

Download PDF
Request Version for Screen Reader
Creator
  • Gracz, Adam D.
    • Affiliation: School of Medicine, Department of Medicine
  • Magness, Scott T.
Abstract
  • The inability to identify, isolate, and culture intestinal epithelial stem cells (IESCs) has been prohibitive to the study and therapeutic utilization of these cells. Using a Sox9EGFP mouse model, we demonstrate that Sox9EGFP fluorescence signatures can be used to differentiate between and enrich for progenitors (Sox9EGFPsubLo) and multipotent IESCs (Sox9EGFPlo). Sox9EGFPlo cells generate “organoids” in a recently defined culture system that mimics the native IESC niche. These organoids possess all four differentiated cell types of the small intestine epithelium, demonstrating the multipotent capacity of Sox9EGFPlo cells. Our results are consistent with the previously reported observation that single IESCs generate cryptlike units without a detectable mesenchymal cell component. A prospective search revealed that CD24 is expressed in the Sox9EGFPlo population and marks IESCs that form organoids in culture. CD24 represents the first cell surface marker that facilitates fluorescence-activated cell sorting enrichment of IESCs with widely available antibodies without requiring a specialized fluorescent reporter gene mouse model.
Date of publication
Keyword
DOI
Identifier
Related resource URL
Resource type
  • Article
Rights statement
  • In Copyright
Journal title
  • Gastrointestinal and Liver Physiology
Journal volume
  • 298
Journal issue
  • 5
Language
  • English
Version
  • Postprint

Relations

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details Sox9 expression marks a subset of CD24-expressing small intestine epithelial stem cells that form organoids in vitro.pdf 2019-05-03 Public Download