ingest cdrApp 2019-01-04T15:05:41.914Z 2a9effba-8beb-4434-9ac1-3dd15c1ac330 modifyDatastreamByValue RELS-EXT fedoraAdmin 2019-01-04T15:06:39.416Z Setting exclusive relation addDatastream MD_TECHNICAL fedoraAdmin 2019-01-04T15:06:52.048Z Adding technical metadata derived by FITS addDatastream MD_FULL_TEXT fedoraAdmin 2019-01-04T15:07:17.118Z Adding full text metadata extracted by Apache Tika modifyDatastreamByValue RELS-EXT fedoraAdmin 2019-01-04T15:07:41.901Z Setting exclusive relation modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2019-01-22T16:03:23.855Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2019-03-20T13:00:49.126Z Allyson Roberts Author Curriculum in Genetics and Molecular Biology School of Medicine Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation. Winter 2018 2018 Cellular biology Developmental biology Apical constriction, END-3, Gastrulation, RNAi eng Master of Science Thesis University of North Carolina at Chapel Hill Graduate School Degree granting institution Genetics and Molecular Biology Bob Goldstein Thesis advisor Janelle Arthur Thesis advisor Bob Duronio Thesis advisor Amy Maddox Thesis advisor Dan McKay Thesis advisor text Allyson Roberts Creator Curriculum in Genetics and Molecular Biology School of Medicine Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation. 2018 2018-12 Cellular biology Developmental biology Apical constriction; END-3; Gastrulation; RNAi eng Master of Science Masters Thesis University of North Carolina at Chapel Hill Graduate School Degree granting institution Genetics and Molecular Biology Bob Goldstein Thesis advisor Janelle Arthur Thesis advisor Bob Duronio Thesis advisor Amy Maddox Thesis advisor Dan McKay Thesis advisor text Allyson Roberts Creator Curriculum in Genetics and Molecular Biology School of Medicine Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation. 2018 2018-12 Cellular biology Developmental biology Apical constriction; END-3; Gastrulation; RNAi eng Master of Science Masters Thesis University of North Carolina at Chapel Hill Graduate School Degree granting institution Bob Goldstein Thesis advisor Janelle Arthur Thesis advisor Bob Duronio Thesis advisor Amy Maddox Thesis advisor Dan McKay Thesis advisor text Roberts_unc_0153M_18251.pdf uuid:1987c05c-0e8c-4766-93d9-09410cf3d59e 2020-12-31T00:00:00 2018-11-29T17:21:34Z proquest application/pdf 1788164