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Allyson
Roberts
Author
Curriculum in Genetics and Molecular Biology
School of Medicine
Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation
There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation.
Winter 2018
2018
Cellular biology
Developmental biology
Apical constriction, END-3, Gastrulation, RNAi
eng
Master of Science
Thesis
University of North Carolina at Chapel Hill Graduate School
Degree granting institution
Genetics and Molecular Biology
Bob
Goldstein
Thesis advisor
Janelle
Arthur
Thesis advisor
Bob
Duronio
Thesis advisor
Amy
Maddox
Thesis advisor
Dan
McKay
Thesis advisor
text
Allyson
Roberts
Creator
Curriculum in Genetics and Molecular Biology
School of Medicine
Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation
There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation.
2018
2018-12
Cellular biology
Developmental biology
Apical constriction; END-3; Gastrulation; RNAi
eng
Master of Science
Masters Thesis
University of North Carolina at Chapel Hill Graduate School
Degree granting institution
Genetics and Molecular Biology
Bob
Goldstein
Thesis advisor
Janelle
Arthur
Thesis advisor
Bob
Duronio
Thesis advisor
Amy
Maddox
Thesis advisor
Dan
McKay
Thesis advisor
text
Allyson
Roberts
Creator
Curriculum in Genetics and Molecular Biology
School of Medicine
Investigation of the molecular mechanisms linking cell-fate specification and shape change in C. elegans gastrulation
There is incredible diversity amongst eukaryotic tissues and overall body plans. Changes in individual cell shapes are essential for generating higher levels of complexity through morphogenetic events. Various molecular mechanisms are responsible for driving cell shape changes during development under tight spatial and temporal regulation. Apical constriction is a process by which cells shrink their apical surfaces through actomyosin contractions to orchestrate tissue-bending events including vertebrate neural tube formation and gastrulation. C. elegans is a valuable system for studying mechanisms that drive apical constriction in vivo, including how one endodermal cell fate-specifying transcription factor, END-3, drives apical constriction of two endodermal cells, thus beginning gastrulation of the embryo. Here, we screened for new gastrulation genes from among genes regulated by END-3 using RNAi and identified 7 new candidate END-3 targets that contribute to gastrulation. We predict that some of these genes may directly or indirectly regulate apical constriction and gastrulation.
2018
2018-12
Cellular biology
Developmental biology
Apical constriction; END-3; Gastrulation; RNAi
eng
Master of Science
Masters Thesis
University of North Carolina at Chapel Hill Graduate School
Degree granting institution
Bob
Goldstein
Thesis advisor
Janelle
Arthur
Thesis advisor
Bob
Duronio
Thesis advisor
Amy
Maddox
Thesis advisor
Dan
McKay
Thesis advisor
text
Roberts_unc_0153M_18251.pdf
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