ingest cdrApp 2017-08-15T22:43:16.528Z d91e81c8-5a8a-4e8a-976c-cad4e396e5ee modifyDatastreamByValue RELS-EXT fedoraAdmin 2017-08-15T22:44:19.874Z Setting exclusive relation modifyDatastreamByValue RELS-EXT fedoraAdmin 2017-08-15T22:44:28.933Z Setting exclusive relation addDatastream MD_TECHNICAL fedoraAdmin 2017-08-15T22:44:38.665Z Adding technical metadata derived by FITS modifyDatastreamByValue RELS-EXT fedoraAdmin 2017-08-15T22:44:56.702Z Setting exclusive relation addDatastream MD_FULL_TEXT fedoraAdmin 2017-08-15T22:45:06.692Z Adding full text metadata extracted by Apache Tika modifyDatastreamByValue RELS-EXT fedoraAdmin 2017-08-15T22:45:16.414Z Setting exclusive relation modifyDatastreamByValue RELS-EXT cdrApp 2017-08-22T13:57:15.627Z Setting exclusive relation modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-01-08T15:58:45.063Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-01-25T08:37:28.277Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-01-27T08:51:42.579Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-03-14T05:26:36.157Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-05-17T17:00:35.655Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-07-11T03:55:15.100Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-07-18T00:12:13.029Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-08-16T13:18:44.775Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-09-21T20:37:30.743Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-09-26T23:58:34.779Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-10-12T00:32:04.309Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2019-03-20T18:28:46.430Z Jennifer Heppert Author Department of Biology College of Arts and Sciences INVESTIGATING MECHANISMS OF MITOTIC SPINDLE POSITIONING The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex Summer 2017 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text Jennifer Heppert Author Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex Summer 2017 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex Summer 2017 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex Summer 2017 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017-08 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation Biology Robert P. Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 University of North Carolina at Chapel Hill Degree granting institution Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR; Fluorescent protein; mitosis; Mitotic spindle eng Doctor of Philosophy Dissertation Biology Robert P. Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 University of North Carolina at Chapel Hill Degree granting institution Jennifer Heppert Creator Department of Biology College of Arts and Sciences Investigating Mechanisms of Mitotic Spindle Positioning The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR, Fluorescent protein, mitosis, Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Robert P. Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Jennifer Heppert Creator Department of Biology College of Arts and Sciences The direction, or orientation, of cell division is important because it determines the fate and positions of cells within a tissue. The position of the mitotic spindle, the molecular machine that separates the chromosomes during mitosis, determines the plane of cell division. Cells sometimes use intercellular signals as spatial cues to position the mitotic spindle, but how mitotic spindles are positioned within cells in response to external cues remains unclear. To approach this question, I used the EMS cell in the early C. elegans embryo, an established model for studying cell interactions and miotic spindle orientation during development. I used contemporary genome editing strategies such as CRISPR, confocal live imaging, and classic embryological techniques, to address how proteins are deployed within cells to position mitotic spindles. The second chapter of this work is an in vivo comparison of fluorescent proteins in C. elegans. This study was a valuable technical advance and revealed which fluorescent proteins to use for in vivo live imaging. In the third chapter, using fluorescent proteins, I created tools to visualize our proteins of interest, and determined whether they were cortically enriched by cell-cell signaling mechanisms to direct mitotic spindle positioning. I found surprisingly, that APC and Dishevelled are enriched asymmetrically at the EMS cortex, but NuMA and dyenin are not. These findings have implications for better understanding how signaling pathway proteins might function as positional cues for spindle orientation, independent of the asymmetric enrichment of the canonical Gα/LGN/NuMA complex 2017 Cellular biology Developmental biology Biology CRISPR; Fluorescent protein; mitosis; Mitotic spindle eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Robert P. Goldstein Thesis advisor Victoria Bautch Thesis advisor Paul Maddox Thesis advisor Michael Major Thesis advisor Stephen Rogers Thesis advisor text 2017-08 Heppert_unc_0153D_17310.pdf uuid:cca78b02-1908-44b6-8fe3-10066e3d52d8 proquest 2019-08-15T00:00:00 2017-07-28T16:02:50Z application/pdf 17241446 yes