ingest cdrApp 2018-06-13T14:47:58.407Z 51cd2fe2-3fd7-401f-a923-a97bc3db68a2 modifyDatastreamByValue RELS-EXT fedoraAdmin 2018-06-13T14:56:43.982Z Setting exclusive relation addDatastream MD_TECHNICAL fedoraAdmin 2018-06-13T14:56:55.759Z Adding technical metadata derived by FITS addDatastream MD_FULL_TEXT fedoraAdmin 2018-06-13T14:57:19.858Z Adding full text metadata extracted by Apache Tika modifyDatastreamByValue RELS-EXT fedoraAdmin 2018-06-13T14:57:20.572Z Setting exclusive relation modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-07-16T21:53:01.596Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-07-18T17:24:56.352Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-08-22T16:10:45.490Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-09-28T19:00:28.619Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2018-10-12T17:48:47.654Z modifyDatastreamByValue MD_DESCRIPTIVE cdrApp 2019-03-22T21:11:46.004Z Rebecca Adikes Author Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Spring 2018 2018 Cellular biology actin, cytoskeleton, microtubule, optogenetics, spectraplakin, XMAP215 eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text Rebecca Adikes Author Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Spring 2018 2018 Cellular biology actin, cytoskeleton, microtubule, optogenetics, spectraplakin, XMAP215 eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text Rebecca Adikes Author Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Spring 2018 2018 Cellular biology actin, cytoskeleton, microtubule, optogenetics, spectraplakin, XMAP215 eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text Rebecca Adikes Author Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Spring 2018 2018 Cellular biology actin, cytoskeleton, microtubule, optogenetics, spectraplakin, XMAP215 eng Doctor of Philosophy Dissertation Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text University of North Carolina at Chapel Hill Degree granting institution Rebecca Adikes Creator Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Cellular biology actin; cytoskeleton; microtubule; optogenetics; spectraplakin; XMAP215 eng Doctor of Philosophy Dissertation Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text University of North Carolina at Chapel Hill Degree granting institution 2018 2018-05 Rebecca Adikes Author Department of Biology College of Arts and Sciences THE REGULATION OF MICROTUBULE DYNAMICS BY +TIPS AND CROSS-LINKING PROTEINS ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 Spring 2018 2018 Cellular biology actin, cytoskeleton, microtubule, optogenetics, spectraplakin, XMAP215 eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Biology Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text Rebecca Adikes Creator Department of Biology College of Arts and Sciences ABSTRACT Rebecca Cressey Adikes: The regulation of microtubule dynamics by +TIPs and cross-linking proteins (Under the supervision of Kevin C. Slep) The dynamic cytoskeleton is composed of distinct filamentous polymer networks. These polymers create highly dynamic scaffolds for many cellular processes in eukaryotes. The polymers provide tracks for cargo transport, mechanically stabilize the cell, establish cell polarity, and are central components of the force-generating machinery required for cell division. In order to perform these various cellular tasks the polymers undergo spatially and temporally regulated assembly and disassembly. Understanding the spatial and temporal regulation of these cellular polymers is essential to understanding cell function. Here, we investigate the spatial and temporal regulation of two polymer networks in cells, the microtubule (MT) and F-actin networks. We developed and employed methods to alter protein targeting and localization using a novel optogenetic approach and a classical structural/functional approach to understand how the recruitment of effectors of these networks alters network dynamics. We developed and employed an optogenetic tool to gain insight into the msec/sec resolution time regime of MT-F-actin cross-linking. This allowed us to uncover key immediate down stream cellular events of cross-linking including decreased MT comet velocities on the sec time scale and an exclusion of MT from the cell periphery on the min-hr time scale. Additionally, using more classical structure/function based experiments, we were able to alter protein targeting using protein domains and through mutation of the protein sequence by just 1-3 2018-05 2018 Cellular biology actin; cytoskeleton; microtubule; optogenetics; spectraplakin; XMAP215 eng Doctor of Philosophy Dissertation University of North Carolina at Chapel Hill Graduate School Degree granting institution Kevin Slep Thesis advisor Kerry Bloom Thesis advisor Bob Goldstein Thesis advisor Paul Maddox Thesis advisor Elizabeth Shank Thesis advisor text Adikes_unc_0153D_17693.pdf uuid:b993e365-7634-4484-a28e-f6800a82f4ea 2020-06-13T00:00:00 2018-04-30T19:51:10Z proquest application/pdf 27958958