Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors
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Alexander, Georgia M, et al. Remote Control of Neuronal Activity In Transgenic Mice Expressing Evolved G Protein-coupled Receptors. 2009. https://doi.org/10.17615/h5rj-v360APA
Alexander, G., Rogan, S., Abbas, A., Armbruster, B., Pei, Y., Allen, J., Nonneman, R., Hartmann, J., Moy, S., Nicolelis, M., Mc Namara, J., & Roth, B. (2009). Remote control of neuronal activity in transgenic mice expressing evolved G protein-coupled receptors. https://doi.org/10.17615/h5rj-v360Chicago
Alexander, Georgia M., Sarah C Rogan, Atheir I Abbas, Blaine N Armbruster, Ying Pei, John A Allen, Randal J Nonneman et al. 2009. Remote Control of Neuronal Activity In Transgenic Mice Expressing Evolved G Protein-Coupled Receptors. https://doi.org/10.17615/h5rj-v360- Creator
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Alexander, Georgia M.
- Other Affiliation: Department of Neurobiology, Duke University, Durham, NC 27710, USA
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Rogan, Sarah C.
- Affiliation: School of Medicine, Department of Pharmacology
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Abbas, Atheir I.
- Other Affiliation: Department of Biochemistry, Case Western Reserve University, Cleveland, OH 44106, USA
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Armbruster, Blaine N.
- Affiliation: School of Medicine, Department of Pharmacology
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Pei, Ying
- Affiliation: School of Medicine, Department of Pharmacology
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Allen, John A.
- Affiliation: School of Medicine, Neurodevelopment Disorders Research Center, Carolina Institute for Developmental Disabilities
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Nonneman, Randal J
- Affiliation: School of Medicine, Neurodevelopment Disorders Research Center, Carolina Institute for Developmental Disabilities
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Hartmann, John
- Affiliation: School of Medicine
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Moy, Sheryl S
- Affiliation: School of Medicine, Neurodevelopment Disorders Research Center, Carolina Institute for Developmental Disabilities
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Nicolelis, Miguel A.
- Other Affiliation: Department of Neurobiology, Duke University, Durham, NC 27710, USA
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McNamara, James O.
- Other Affiliation: Department of Neurobiology, Duke University, Durham, NC 27710, USA
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Roth, Bryan
- ORCID: https://orcid.org/0000-0002-0561-6536
- Affiliation: Eshelman School of Pharmacy, Division of Chemical Biology and Medicinal Chemistry, N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
- Other Affiliation: National Institute of Mental Health Psychoactive Drug Screening Program, Duke University, Durham, NC 27710, USA
- Abstract
- Examining the behavioral consequences of selective CNS neuronal activation is a powerful tool for elucidating mammalian brain function in health and disease. Newly developed genetic, pharmacological, and optical tools allow activation of neurons with exquisite spatiotemporal resolution; however, the inaccessibility to light of widely distributed neuronal populations and the invasiveness required for activation by light or infused ligands limit the utility of these methods. To overcome these barriers, we created transgenic mice expressing an evolved G protein-coupled receptor (hM3Dq) selectively activated by the pharmacologically inert, orally bioavailable drug clozapine-N-oxide (CNO). Here, we expressed hM3Dq in forebrain principal neurons. Local field potential and single-neuron recordings revealed that peripheral administration of CNO activated hippocampal neurons selectively in hM3Dq-expressing mice. Behavioral correlates of neuronal activation included increased locomotion, stereotypy, and limbic seizures. These results demonstrate a powerful chemical-genetic tool for remotely controlling the activity of discrete populations of neurons in vivo.
- Date of publication
- 2009
- DOI
- Identifier
- 2-s2.0-68349103200
- https://doi.org/10.1016/j.neuron.2009.06.014
- Related resource URL
- Resource type
- Article
- Rights statement
- In Copyright
- Journal title
- Neuron
- Journal volume
- 63
- Journal issue
- 1
- Page start
- 27
- Page end
- 39
- Language
- English
- Version
- Postprint
- ISSN
- 0896-6273
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