Molecular dosimetry of n7-guanine adducts in male and female mice exposed to 1,3-butadiene by inhalation

Public Deposited
Analytics

Downloadable Content

Download PDF
Request Version for Screen Reader
Last Modified
  • March 21, 2019
Creator
  • Troutman, John M.
    • Affiliation: School of Medicine, Curriculum in Toxicology
Abstract
  • 1,3-Butadiene (BD) is a well-characterized carcinogen that is both an occupational and environmental hazard and an important industrial chemical widely used in the production of rubber and plastic and is also present in automobile exhaust and cigarette smoke. Upon metabolic activation in vivo, it forms three epoxides that can react with nucleophilic sites in biomolecules: 1,2,3,4-diepoxybutane (DEB), 1,2-epoxy-3-butene (EB), and 3,4-epoxy-1,2-butane-diol (EBD). We characterized the molecular dosimetry of N-7 guanine adduct formation by these metabolites of BD in the liver of male and female B6C3F1 mice exposed to varying levels of BD. The adducts, racemic and meso forms of N-7-(2,3,4-trihydroxy-3-buten-2-yl)guanine (THB-Gua), N-7-(2-hydroxy-3-buten-1-yl)guanine (HB-Gua I), and N-7-(1-hydroxy-3-buten-2-yl)guanine (HB-Gua II) were similar in male and female B6C3F1 mice at all levels where liver tissue was available for both sexes. Thus, no gender differences in adduct formation were noted. The THB-Gua adducts were the most abundant and exhibited a supralinear dose response.
Date of publication
DOI
Resource type
Rights statement
  • In Copyright
Note
  • "... in partial fulfillment of the requirements for the degree of Master of Science in the Curriculum in Toxicology."
Advisor
  • Swenberg, James A.
Language
Publisher
Place of publication
  • Chapel Hill, NC
Access right
  • Open access
Date uploaded
  • March 18, 2013

Relations

Parents:

This work has no parents.

Items

Thumbnail Title Date Uploaded Visibility Actions
file details 2019-04-11 Public Download