Cell and Matrix Interactions during Branching Morphogenesis
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Harunaga, Jill. Cell and Matrix Interactions During Branching Morphogenesis. Chapel Hill, NC: University of North Carolina at Chapel Hill Graduate School, 2014. https://doi.org/10.17615/gky7-h064APA
Harunaga, J. (2014). Cell and Matrix Interactions during Branching Morphogenesis. Chapel Hill, NC: University of North Carolina at Chapel Hill Graduate School. https://doi.org/10.17615/gky7-h064Chicago
Harunaga, Jill. 2014. Cell and Matrix Interactions During Branching Morphogenesis. Chapel Hill, NC: University of North Carolina at Chapel Hill Graduate School. https://doi.org/10.17615/gky7-h064- Last Modified
- March 19, 2019
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Harunaga, Jill
- Affiliation: College of Arts and Sciences, Department of Biology
- Abstract
- During embryonic development when tissues are particularly plastic, cells within a tissue can often interact with their surrounding extracellular matrix in a reciprocal manner: the cells remodel the matrix, but the matrix can induce signaling and changes in cell behavior, which in turn can affect matrix remodeling to sculpt tissue architecture. A specialized type of extracellular matrix is the basement membrane, which underlies or encapsulates epithelial tissues. We have used the embryonic mouse salivary gland as a model to study cell-basement membrane interactions during branching morphogenesis. We first focused on whether the cells in contact with the basement membrane (termed outer bud cells) behave differently from cells that remain in contact only with other epithelial cells (inner bud cells). Using a transgenic mouse expressing a photoconvertible fluorescent probe to optically highlight small populations of cells within developing salivary glands, we tracked their migration. The outer cells migrated much more rapidly than the inner cells and each cell population required different proteins for their migration. Therefore, there are two distinct populations of epithelial cells that utilize two different modes of migration in the salivary gland. We also found that the basement membrane was remarkably dynamic, being remodeled on a local and global scale. There are hundreds of tiny perforations in the basement membrane surrounding the tips of rapidly expanding end buds in embryonic lung, kidney, and salivary gland. The entire basement membrane also translocates rearward and accumulates to stabilize the duct. Both the micro-perforations and translocation are dependent on myosin II and protease activity. We speculate that the micro-perforations locally increase the distensibility of the basement membrane, allowing directed expansion of the epithelium and basement membrane translocation. Interestingly, the perforations could also allow increased epithelial cell exposure to the mesenchyme, which could stimulate the motility of the outer cells. In summary, we have described a dynamic, bidirectional system in which the cells modify the basement membrane, which in turn affects cell behavior and matrix remodeling to sculpt tissue architecture during branching of the embryonic mouse salivary gland.
- Date of publication
- August 2014
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- In Copyright
- Advisor
- Peifer, Mark
- Adelstein, Robert
- Rogers, Stephen
- Yamada, Kenneth
- Goldstein, Bob
- Bloom, Kerry
- Degree
- Doctor of Philosophy
- Degree granting institution
- University of North Carolina at Chapel Hill Graduate School
- Graduation year
- 2014
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- Place of publication
- Chapel Hill, NC
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- There are no restrictions to this item.
- Date uploaded
- April 23, 2015
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