Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR
Public DepositedAdd to collection
You do not have access to any existing collections. You may create a new collection.
Downloadable Content
Download PDFCitation
MLA
Seaman, William T, et al. Detection and Quantitation of Hpv In Genital and Oral Tissues and Fluids by Real Time Pcr. BioMed Central Ltd, 2010. https://doi.org/10.17615/xjp3-ca50APA
Seaman, W., Andrews, E., Couch, M., Kojic, E., Cu Uvin, S., Palefsky, J., Deal, A., & Webster Cyriaque, J. (2010). Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR. BioMed Central Ltd. https://doi.org/10.17615/xjp3-ca50Chicago
Seaman, William T, Elizabeth Andrews, Marion Couch, Erna M Kojic, Susan Cu Uvin, Joel Palefsky, Allison M Deal et al. 2010. Detection and Quantitation of Hpv In Genital and Oral Tissues and Fluids by Real Time Pcr. BioMed Central Ltd. https://doi.org/10.17615/xjp3-ca50- Creator
-
Seaman, William T
- Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
-
Andrews, Elizabeth
- Affiliation: School of Dentistry, Department of Dental Ecology
- Other Affiliation: College of Dental Medicine, Western University of Health Sciences, Pomona, CA, USA
-
Couch, Marion
- Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
-
Kojic, Erna M
- Other Affiliation: Department of Medicine, The Miriam Hospital, Brown University, Providence, RI, USA
-
Cu-Uvin, Susan
- Other Affiliation: Department of Medicine, The Miriam Hospital, Brown University, Providence, RI, USA
-
Palefsky, Joel
- Other Affiliation: Department of Medicine, University of California, San Francisco, San Francisco, CA, USA
-
Deal, Allison M
- Affiliation: N.C. Cancer Hospital, UNC Lineberger Comprehensive Cancer Center
-
Webster-Cyriaque, Jennifer
- Affiliation: School of Medicine, Department of Microbiology and Immunology, School of Dentistry, Department of Dental Ecology
- Abstract
- Abstract Background Human papillomaviruses (HPVs) remain a serious world health problem due to their association with anogenital/oral cancers and warts. While over 100 HPV types have been identified, a subset is associated with malignancy. HPV16 and 18 are the most prevalent oncogenic types, while HPV6 and 11 are most commonly responsible for anogenital warts. While other quantitative PCR (qPCR) assays detect oncogenic HPV, there is no single tube assay distinguishing the most frequent oncogenic types and the most common types found in warts. Results A Sybr Green-based qPCR assay was developed utilizing degenerate primers to the highly conserved HPV E1 theoretically detecting any HPV type. A single tube multiplex qPCR assay was also developed using type-specific primer pairs and TaqMan probes that allowed for detection and quantitation of HPV6,11,16,18. Each HPV type was detected over a range from 2 × 101 to 2 × 106copies/reaction providing a reliable method of quantitating type-specific HPV in 140 anogenital/cutaneous/oral benign and malignant specimens. 35 oncogenic and low risk alpha genus HPV types were detected. Concordance was detected in previously typed specimens. Comparisons to the gold standard detected an overall sensitivity of 89% (95% CI: 77% - 96%) and specificity of 90% (95%CI: 52% - 98%). Conclusion There was good agreement between the ability of the qPCR assays described here to identify HPV types in malignancies previously typed using standard methods. These novel qPCR assays will allow rapid detection and quantitation of HPVs to assess their role in viral pathogenesis.
- Date of publication
- August 19, 2010
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- William T Seaman et al.; licensee BioMed Central Ltd.
- License
- Journal title
- Virology Journal
- Journal volume
- 7
- Journal issue
- 1
- Page start
- 194
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1743-422X
- Bibliographic citation
- Virology Journal. 2010 Aug 19;7(1):194
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- August 23, 2012
Relations
- Parents:
This work has no parents.
Items
Thumbnail | Title | Date Uploaded | Visibility | Actions |
---|---|---|---|---|
1743-422X-7-194.pdf | 2019-05-06 | Public | Download | |
1743-422X-7-194.xml | 2019-05-06 | Public | Download |