LCN6, a novel human epididymal lipocalin
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Hamil, Katherine G, et al. Lcn6, a Novel Human Epididymal Lipocalin. BioMed Central Ltd, 2003. https://doi.org/10.17615/s9ac-d926APA
Hamil, K., Liu, Q., Sivashanmugam, P., Anbalagan, M., Yenugu, S., Soundararajan, R., Grossman, G., Rao, A., Birse, C., Ruben, S., Richardson, R., Zhang, Y., O'rand, M., Petrusz, P., French, F., & Hall, S. (2003). LCN6, a novel human epididymal lipocalin. BioMed Central Ltd. https://doi.org/10.17615/s9ac-d926Chicago
Hamil, Katherine G, Qiang Liu, P Sivashanmugam, M Anbalagan, Suresh Yenugu, Rama Soundararajan, Gail Grossman et al. 2003. Lcn6, a Novel Human Epididymal Lipocalin. BioMed Central Ltd. https://doi.org/10.17615/s9ac-d926- Creator
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Hamil, Katherine G
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Laboratories for Reproductive Biology
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Liu, Qiang
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Shanghai Institutes for Biological Science
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Sivashanmugam, P
- Affiliation: School of Medicine, Department of Cell Biology and Physiology
- Other Affiliation: Duke University
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Anbalagan, M
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Indian Institute of Science
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Yenugu, Suresh
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Laboratories for Reproductive Biology
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Soundararajan, Rama
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: University of California, San Francisco
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Grossman, Gail
- Affiliation: School of Medicine, Department of Cell Biology and Physiology
- Other Affiliation: Laboratories for Reproductive Biology
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Rao, AJ
- Other Affiliation: Indian Institute of Science
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Birse, Charles E
- Other Affiliation: Human Genome Sciences, Inc
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Ruben, Stephen M
- Other Affiliation: Human Genome Sciences, Inc
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Richardson, Richard T
- Affiliation: School of Medicine, Department of Cell Biology and Physiology
- Other Affiliation: Laboratories for Reproductive Biology
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Zhang, Yong-Lian
- Other Affiliation: Shanghai Institutes for Biological Sciences
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O'Rand, Michael G
- Affiliation: School of Medicine, Department of Cell Biology and Physiology
- Other Affiliation: Laboratories for Reproductive Biology
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Petrusz, Peter
- Affiliation: School of Medicine, Department of Cell Biology and Physiology
- Other Affiliation: Laboratories for Reproductive Biology
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French, Frank S
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Laboratories for Reproductive Biology
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Hall, Susan H
- Affiliation: School of Medicine, Department of Pediatrics
- Other Affiliation: Laboratories for Reproductive Biology
- Abstract
- Abstract Background The lipocalin (LCN) family of structurally conserved hydrophobic ligand binding proteins is represented in all major taxonomic groups from prokaryotes to primates. The importance of lipocalins in reproduction and the similarity to known epididymal lipocalins prompted us to characterize the novel human epididymal LCN6. Methods and Results LCN6 cDNA was identified by database analysis in a comprehensive human library sequencing program. Macaca mulatta (rhesus monkey) cDNA was obtained from an epididymis cDNA library and is 93% homologous to the human. The gene is located on chromosome 9q34 adjacent LCN8 and LCN5. LCN6 amino acid sequence is most closely related to LCN5, but the LCN6 beta-barrel structure is best modeled on mouse major urinary protein 1, a pheromone binding protein. Northern blot analysis of RNAs isolated from 25 human tissues revealed predominant expression of a 1.0 kb mRNA in the epididymis. No other transcript was detected except for weak expression of a larger hybridizing mRNA in urinary bladder. Northern hybridization analysis of LCN6 mRNA expression in sham-operated, castrated and testosterone replaced rhesus monkeys suggests mRNA levels are little affected 6 days after castration. Immunohistochemical staining revealed that LCN6 protein is abundant in the caput epithelium and lumen. Immunofluorescent staining of human spermatozoa shows LCN6 located on the head and tail of spermatozoa with the highest concentration of LCN6 on the post-acrosomal region of the head, where it appeared aggregated into large patches. Conclusions LCN6 is a novel lipocalin closely related to Lcn5 and Lcn8 and these three genes are likely products of gene duplication events that predate rodent-primate divergence. Predominant expression in the epididymis and location on sperm surface are consistent with a role for LCN6 in male fertility.
- Date of publication
- November 14, 2003
- DOI
- Identifier
- Resource type
- Article
- Rights statement
- In Copyright
- Rights holder
- Katherine G Hamil et al.; licensee BioMed Central Ltd.
- Journal title
- Reproductive Biology and Endocrinology
- Journal volume
- 1
- Journal issue
- 1
- Page start
- 112
- Language
- English
- Is the article or chapter peer-reviewed?
- Yes
- ISSN
- 1477-7827
- Bibliographic citation
- Reproductive Biology and Endocrinology. 2003 Nov 14;1(1):112
- Publisher
- BioMed Central Ltd
- Access right
- Open Access
- Date uploaded
- September 5, 2012
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