Selective Classification of Citrullination and Acetylation on Histone Tails via Organic Receptor Molecules
Public Deposited- Last Modified
- February 26, 2019
- Creator
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Ramsay, Christopher
- Affiliation: College of Arts and Sciences, Department of Chemistry
- Abstract
- The goal of this research is to determine whether an indicator displacement assay using organic small-molecule receptors and a fluorescent dye lucigenin (LCG) can correctly classify citrullination and acetylation in the context of histone tail peptides. Difference in fluorescent signal, dictated by the displacement of receptor-bound LCG, will be the benchmark by which the post-translational modifications (PTMs) of interest will be classified. Linear discrimination analysis (LDA) will provide the statistical means to visually separate and classify the fluorescence data for several species into respective categories. The proposed assay schema was shown to be effective in differentiating similarly citrullinated and acetylated peptides at low concentrations (15 μM) and was shown to be sensitive enough for 10 μM in citrullinated H3 tail peptides and 5 μM in acetylated H3 tail peptides.
- Date of publication
- spring 2015
- Keyword
- DOI
- Resource type
- Rights statement
- In Copyright
- Note
- Funding: Carrie Largent Summer Research Fellowship
- Advisor
- Waters, Marcey
- Degree
- Bachelor of Arts
- Honors level
- Honors
- Degree granting institution
- University of North Carolina at Chapel Hill
- Extent
- 14
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