Anterior Cruciate Ligament (ACL) injuries occur frequently in athletics, and plyometric exercises may aid in preventing these injuries. The purpose of this study was to determine which plyometric exercises produce the greatest activation of the gluteal and hamstrings muscles and the medial-to-lateral hamstrings activation ratio. Forty-one subjects performed 5 plyometric exercises while muscle activity was recorded. Subjects displayed the most hamstrings and gluteal muscle activity during the single-leg sagittal plane hurdle hops, the least muscle activity during the 180o hops, and the greatest preparatory medial-to-lateral hamstrings ratio during the double-leg sagittal plane hurdle hops and split squat jumps. Therefore, 180o hops are not as effective as other plyometric exercises at targeting gluteal and hamstrings activation. Frontal or sagittal plane exercises are needed to enhance medial-to-lateral hamstrings activation and gluteal activation, respectively. Future research should examine the effects of frontal versus sagittal plane plyometric exercise intervention programs on knee biomechanics.