The human papillomavirus (HPV) vaccination literature supports the role of culture on African-Americans’ HPV vaccination acceptance. However, the evidence supporting the role of culture on this health behavior is limited. Few studies examine the influence of culture on HPV vaccination acceptance among African-Americans using theoretical frameworks grounded in culture, and few studies examine the influence of culture from the perspective of adolescent females. Therefore, using a dyadic approach, this dissertation explored the role of culture on African-American parents’ and their adolescent daughters’ (12 to 17 years old) HPV vaccination acceptance using both the PEN-3 cultural model and the Health Belief Model. Grounded theory techniques and quantitative descriptive statistics were used to explore the cultural factors that influence the HPV vaccine acceptance of 29 African-American parent-daughter dyads (n = 30 parents; n = 34 daughters) residing in the Southeastern and Northeastern United States. This dissertation’s findings provide evidence of the role of culture on African-American parents’ and their adolescent daughters’ HPV vaccine acceptance, and suggest the need for future research to use a culturally empowering lens to better understand African-American parents’ and adolescent daughters’ cervical cancer prevention health behaviors. Additionally, the findings support the usefulness of using a culture-centered theory to explore culture-related factors that influence the HPV vaccine acceptance of African-American parents and daughters. Incorporating a dyadic approach was also useful for understanding how cultural factors that influence African-American parents’ and daughters’ perceptions and health behaviors may be transmitted among families, kinship networks, and the community. The findings from this dissertation elucidate new areas that may potentially inform the development of culturally appropriate interventions to advance the field of cervical cancer prevention research.