Comparative Mutagenicity of Urinary Metabolites of Nitropolycyclic Aromatic Hydrocarbons 1-Nitropyrene, 2-Nitrofluoranthene, and 3-Nitrofluoranthene
Public Deposited- Last Modified
- February 27, 2019
- Creator
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Boucher, Michael N.
- Affiliation: Gillings School of Global Public Health, Department of Environmental Sciences and Engineering
- Abstract
- This study compared the mutagenicity of urinary metabolites of prevalent air pollutants 1-nitropyrene (1-NP), 2-nitrofluoranthene (2-NFA), and 3-nitrofluoranthene (3-NFA). Rats were injected intra-peritoneally with specific doses and their urine was analyzed for mutagenicity by an Ames plate incorporation assay using Salmonella Typhimurium. Resulting data yielded revertant excretion rate plots used for comparative analysis. To achieve maximum mutagenicity, 1-NP's urinary metabolites required exogenous activation by S9, 2-NFA's did not vary significantly with S9 activation, while 3-NFA's was decreased by S9 metabolism. 2-NFA's mutagenic metabolites' revertant excretion rate was a quarter of the rates of 3-NFA's and 1-NP's mutagenic metabolites. The strongest mutagenicity of urinary metabolites during the 48 hrs after injection was from 1-NP which was double the amounts from 3-NFA and 2-NFA. However, the estimated total urinary mutagenicity showed 1-NP and 2-NFA potentially created equal amounts while 3-NFA's amount was only half 1-NP's amount. All three compounds' urinary mutagen excretion rates are formation rate limited.
- Date of publication
- May 1991
- DOI
- Resource type
- Rights statement
- In Copyright
- Advisor
- Ball, Louise
- Todd, Lori A.
- Reist, Parker C.
- Degree
- Master of Science in Public Health
- Academic concentration
- Environmental Health Sciences
- Degree granting institution
- University of North Carolina at Chapel Hill
- Graduation year
- 1991
- Language
- Deposit record
- ad7e9272-7c4f-43e0-afeb-7a5f2526ad76
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